The integration of a chemical lysis step in another microfluidic chip enabled the quantification of a range of membrane-associated (EpCAM, α-IGF-1R, CA125, CD9, CD81, and CD63) and internal (e.g. phosphorylated type 1 insulin growth factor receptor, IGF-1R) EV proteins from two lung cancer patients, two ovarian cancer patients, and healthy controls [49]. This evidence concerns the gene CD63 and ovarian cancer.