For this, the colon cancer cell lines HT-29 and HCT116 were treated with CCL20 and culture supernatants were checked for the secretion of classical cancer cell growth factors such as epidermal growth factor (EGF), fibroblast growth factor (FGF), hepatocyte growth factor (HGF), insulin-like growth factor (IGF), platelet-derived growth factor (PDGF), transforming growth factor alpha (TGF-α), and amphiregulin by ELISA. The gene discussed is AREG; the disease is malignant colon neoplasm.