TGFA and colorectal cancer: We next assessed for a possible role of TGF-α or amphiregulin in CCL20-dependent colorectal cancer cell migration, CCL20 production, or proliferation as secretion of these factors in response to CCL20 was seen in some of our assays, and as a prior study showed that CCL20-dependent proliferation in the CaCo-2 colorectal cancer cells was dependent on amphiregulin secretion.[13] In contrast to what was seen for HGF, TGF-α and amphiregulin did not appear to play a role in CCL20-dependent colorectal cancer cell migration, CCL20 production, or proliferation.