The panel of F. nucleatum+pks+ E. coli+CEA+CA19-9+FOBT (panel 5) was chosen as optimal panel in differentiating CRC from the controls, with an AUC of 0.887 (95% CI 0.68 to 1.0), sensitivity of 75.0%, specificity of 98.1%, NPV of 0.98, and PPV of 0.77. The gene discussed is ARAF; the disease is colorectal carcinoma.