GTF2E2 and esophageal squamous cell carcinoma: Then, we investigated the expression levels of GTF2E2 in several ESCC cell lines with RT-PCR and western blot analysis normalized to the normal esophageal epithelial cell line, HEEC (Fig. 1H-I), and the results indicated that GTF2E2 was overexpressed in our examined ESCC cell lines.