As shown in Fig. 1D, there is no significant difference between NF-CM groups and control groups whereas MDA-MB-231 breast cells co-cultured with CAF-CM showed increased expression of β‐catenin, N-cadherin, MMP-9, and MMP-3, indicating that CAF-CM elevated the migration and invasion capacity of the breast cancer cells. This evidence concerns the gene MMP3 and breast carcinoma.