To determine whether CAFs is related to EMT of breast cancer cells, western blot results showed that MDA-MB-231 cells co-cultured with CAFs had the lower expression of E-cadherin and higher expression of N-cadherin and Vimentin than that of MDA-MB-231 cells co-cultured with NFs and we got similar results from MCF-7 cells (Fig. 6A, right). The gene discussed is VIM; the disease is breast cancer.