To further investigate whether ANKRD52 or other immune evasion mutations participate in cancer sensitivity to T cell-mediated cytotoxicity directly, we performed a parallel in vitro CRISPR screen whereas MC38 cells expressing ovalbumin (MC38-OVA) were introduced with the mMCE sgRNA library and then co-cultured with antigen-specific OT-I T cells (Fig. 3a, b; Supplementary Fig. 6a, b). Here, ANKRD52 is linked to cancer.