A series of genes such as EN1, S100A4, ANXA1, PDPN, IGFBP2 and CHI3L1 were upregulated in radiotherapy treated glioma patients, while other genes such as PRLHR, SFRP2, BRINP1, TRIM67, LHX5, KCNIP2 and NSG2 were downregulated (S1 Table). This evidence concerns the gene EN1 and central nervous system cancer.