Given the essential roles of ESCRT proteins and ISG15, we characterized the functions of ESCRT proteins, including TSG101, ALIX, and CHMP4A; the roles of HERC5 and ISG15; during TBFV infection using an array of TBFV (LGTV, TBEVSofjin, KFDV, and POWV) and compared these with MBFV (WNV Kunjin strain [WNVKunjin] and ZIKV). Here, ISG15 is linked to infection.