However, after 18 weeks of feeding, ULK1 expression and LC3 lipidation were decreased in the livers from HFD-fed Apoe−/− mice, suggesting autophagy inhibition, a feature previously described in advanced stages of NAFLD (Ding et al., 2020; Lee et al., 2020; Lu et al., 2020). This evidence concerns the gene ULK1 and metabolic dysfunction-associated steatotic liver disease.