TP53 and Peutz-Jeghers syndrome: To provide an orthogonal read-out of STK11 function generated by our in vitro autophosphorylation activity assay we next took advantage of an STK11-dependent, p53-mediated transcriptional activation assay previously published to evaluate the pathogenicity of STK11 germline mutations associated with Peutz-Jeghers Syndrome (27,40,41).