TP53 showed the highest frequency of pathogenic variants, with variants found in 10 tumors [p.Arg273His/c.818 G>A (in 2 gliomas), p.Arg273Cys/c.817 C>T, p.Arg175His/c.524 G>A (in 2 gliomas), p.Arg248Gly/c.742 C>G, p.Arg342*/c.1024 C>T, p.Arg248Trp/c.742 C>T, p.Val173Leu/c.517 G>T, p.Arg213Gln/c.638 G>A, p.Gly245Ser/c.733 G>A, p.Arg213*/c.637 C>T, p.Arg267Trp, c.799 C>T, p.Arg248Gln, c.743 G>A]. The gene discussed is TP53; the disease is glioma.