Associations were observed at six previously described autosomal non-syndromal hearing loss loci: DFNA10 (deafness autosomal dominant 10), which contains the gene EYA4 (eyes absent homolog 4); DFNB28 (deafness autosomal recessive 28), which contains TRIOBP (TRIO and F-actin-binding protein); DFNB61, which contains the gene that encodes prestin, SLC26A5; DFNA58, DFNB47, and DFNB79, which contains TPRN (taperin) and CACNA1B (voltage-dependent N-type calcium channel subunit alpha-1B, Additional file 1: Table 3). This evidence concerns the gene EYA4 and hearing loss disorder.