We found that loss of NUCKS1 reduces SKP2 gene expression across a cell line panel comprising three non-cancer cell lines (hTERT-immortalised bronchial epithelial cells: NBE1-hTERT; normal primary embryonic fibroblasts: TIG-1; hTERT-immortalised retinal epithelial cells: RPE1-hTERT), and six cancer cell lines (five colorectal cancer cell lines: HCT116, RKO, HT29, DLD1, CACO2; and osteosarcoma cells: U2OS) (Fig. 1d, Supplementary Fig. 1E). The gene discussed is SKP2; the disease is osteosarcoma.