Afterwards, this polypeptide hydrogel tumor vaccine delivery system can be prepared by simply mixing tumor cell lysates (TCL) prepared by B16 tumor cells and TLR3 agonist polyinosinic: polycytidylic acid (poly (I:C)) with PEV copolymer in aqueous solution, which can continuously release antigens or poly (I:C) for more than one week, prolong the antigen retention time at the injection site, and increase the percentage of antigen drainage to lymph nodes. The gene discussed is TLR3; the disease is neoplasm.