In order to monitor in vivo‐like kidney damage, secretion of kidney injury molecule‐1 (KIM‐1) as an indicator of acute renal failure was significantly increased in the supernatants from kidney organoids exposed to Stx2a compared to supernatants from intoxicated organoids pre‐treated with OSMI‐1 (Fig 6C), suggesting that O‐GlcNAc inhibition effectively reduces Stx2a‐induced renal injury. This evidence concerns the gene STX2 and acute kidney injury.