There was increase and decrease in SPI1 enrichment, promoter‐luciferase reporter activity, as well as levels of HK2 and PGK1 in cancer cells stably over‐expressing or silencing SPIB, which was eliminated by silencing or ectopic expression of SPI1, respectively (Figures 6B–E and S7A–C). This evidence concerns the gene HK2 and cancer.