HK2 and cancer: On the other hand, co‐culture with TANs led to increase of SPI1 expression and its enrichment on HK2 or PGK1 promoter in LoVo and HCT116 cells (Figures 3B, S3F and S4A), resulting in increase of HK2 or PGK1 promoter activity and transcript levels (Figure S4B, C), while silencing of SPI1 in cancer cells reduced these effects (Figures 3C and S4A–C).