In order to determine the specific way that NUDT1 affects the stability of SIRT3, lysosomal inhibitor chloroquine and proteasome inhibitor MG132 were added to the ccRCC cell lines with stable knockdown of NUDT1, and the expression of SIRT3 was detected by Western blotting. Here, NUDT1 is linked to nonpapillary renal cell carcinoma.