Analogously, in order to determine the effects of RPS15A and TMED3 on osteosarcoma, experimental groups of TMED3 + NC-shRPS15A, shRPS15A + NC-TMED3, TMED3 + shRPS15A and shRPS15A + shTMED3 were established in MNNG/HOS cells (Additional file 3: Fig. S3B–D). Here, RPS15A is linked to osteosarcoma.