VEGFA and hypospadias: The qPCR analyses showed decreasing of VEGF expression in both distal and proximal (− 1.05 (− 0.1–(− 1.9)); − 1 (− 0.2–(− 2.3))) hypospadias compared with the control group − 0.8 (− 0.4–(− 1.1)), however were not significantly different.