We also used immunohistochemical analysis method to show IGF2, p-IGF1R, p-Akt (S473) and PTEN expression in tumor tissues in the validation cohort (Figure 4B), the results revealed that the level of IGF2 and phosphoprotein were increased in the poor response group compared to good response group, but PTEN expression was just the opposite (Table 3). This evidence concerns the gene PTEN and neoplasm.