In addition,PRP contains different growth factors, such as PDGF,transforming growth factor β (TGF-β), VEGF, epidermalgrowth factor (EGF), insulin-like growth factor (IGF) andfibroblast growth factor (FGF), which have been appliedto treat androgenic alopecia and chronic wounds (23, 24).Despite the promising results of using stem cell-derivedconditioned media (CM) in treating different diseases, theshort half-life of cytokines and growth factors present inCM is a disadvantage for regenerative therapy (25). This evidence concerns the gene VEGFA and androgenetic alopecia.