ANXA5 and plasma cell myeloma: The LentimiRa-off-has-miR-19a-3p transfected myeloma cells wereincubated for 48 hours with BTZ (5 μM for U266 and 150 nMfor RPMI 8226) followed by annexin V/PI staining and flowcytometry analysis to determine the percentage of apoptosis.The percentage of cells that underwent apoptosis increasedafter transfection in the RPMI 8226 (23.5% vs. 68.2%,P=0.0038) and U266 (25.2% vs. 96.4%, P=0.0006) cell linescompared with the non-transfected cell lines and the negativecontrol (Fig .4A-D).