Thus, both ovarian cancer cell lines PA1 and A2780 were treated with 10, 20, and 40 nM of ST09 drug for 48 h and the expression of pro-survival factor BCL-2, pro-apoptotic proteins Bax and Bak, cytochrome C, apoptosome complex protease Apaf-1, and cleaved caspase 9 were evaluated. Here, APAF1 is linked to ovarian cancer.