PTPRC and glioblastoma: To understand how neo-aPD1 changes the immune landscape of the tumor microenvironment, we used CyTOF mass cytometry (CyTOF), single-cell RNA sequencing (scRNAseq), and/or multiplex immunofluorescence (mIF) to analyze the tumor-infiltrating CD45+ immune cells from a total of 69 unique GBM patients (Fig. 1a, b).