GFAP and ischemic stroke: As shown in Fig. 6, after 14 days of ischemic stroke, both immunolabeled GFAP+ reactive astrocytes and Iba-1+ microglia/macrophages exhibited phagocytic inclusions, including synapses and myelin-like structures within their cytoplasm, indicating that microglia/macrophages and astrocytes actively engulf synapses.