Next, we examined the expression of these genes in another cohort using real-time quantitative PCR and the results also showed that both RNA methyltransferases (METTL3, METTL14, and WTAP) and demethylases (FTO and ALKBH5) were significantly downregulated in MDD (Fig. 1b). The gene discussed is ALKBH5; the disease is major depressive disorder.