ELN and type 2 diabetes mellitus: To confirm vascular fibrosis, elastin fibers were stained bright red with Orcein staining (Figure 7A) The thoracic aorta of T2DM mice had increased inner diameter (cross-sectional area of aorta, Figure 8(Ba)) and intimal thickness (length of tunica intima–media, Figure 8(Bb)) compared to controls.