Conversely, in a cohort study of 51 children, Koot et al., did not find any relationship between PNPLA3 rs738409 SNP and liver steatosis improvement in a 6-month intensive lifestyle treatment [72], and neither PNPLA3 nor TM6F2 variants were related to NAFLD improvement after a 4-month reduction of caloric intake, although these risk genotypes did not impair the response of dietetic intervention [73]. This evidence concerns the gene PNPLA3 and fatty liver disease.