Additionally, PAS-positive area was only different in the single-insult CVB4 (V(+)ND) animals, while kidney weight and Tgfb1 expression differed in the single-insult T1D (V(−)DB) animals, and body weight in the double-insult (V(+)DB) group. This evidence concerns the gene TGFB1 and type 1 diabetes mellitus.