In the experiment with BCG-prime → Flu/THSP vector boost immunization followed by M. tuberculosis H37Rv challenge infection we have shown that the T-cellular antigen-specific immune response on restimulation with TB10.4/HspX protein mix was mediated by IFNγ+ and IFNγ+TNFα+ cells (both CD4+ and CD8+). The gene discussed is IFNG; the disease is infection.