The general plan of the in vitro research was to establish a 3D model to evaluate whether the SFV/IFNg vector, through infection of 4T1 mouse breast cancer cells, can induce a tumor suppressive phenotype in macrophages (M1), followed by the assessment of the therapeutic potential of the SFV/IFNg vector in vivo. The gene discussed is IFNG; the disease is breast carcinoma.