BCL2L1 and infection: To investigate the relationship between AdBcl-xL and GSIV-induced apoptosis, GSM cells transfected with pCDNA3.1-flag-Bcl-xL or pCDNA3.1-flag and non-transfected cells, following GSIV infection, were stained with annexin v and PI and analyzed by flow cytometry (Figure 3A).