LINS1 and infection: As shown in Figure 1, vaccination with the Ad-liNS1/NS2 vaccine resulted in significantly lower viral loads in the brain of vaccinated mice compared to naive mice on day 7 post i.c. Nevertheless, the weight loss observed beyond day 5 after viral challenge suggested that the viral loads in the vaccinated mice peaked at a higher peak level compared to mice immunized through prior infection (the ZIKV immune control group) whose weights were unaffected following i.c. challenge.