To confirm the possibility that infectivity of the rD/OK-8seg was induced by co-infection with two or more non-infectious viruses possessing incomplete genome sets, we independently generated seven-segmented recombinant viruses either with NS1 or NS2 segments by reverse genetics under the same rescue conditions, and co-infected the cells with these two non-infectious viruses. Here, LZTR1 is linked to coinfection.