PTX treatment did not prevent death caused by ZIKV infection in SH-SY5Y cells, as quantified by LIVE/DEAD staining followed by FCM analysis (Supplemental Figure S4E,F), suggesting that TNF-α synthesis does not seem to be determinant for ZIKV-induced apoptosis in SH-SY5Y cells. This evidence concerns the gene TNF and Zika virus infectious disease.