Bearing in mind the increasing interest in low-grade inflammation and its role in the development of a wide range of inflammation-related disorders, we purposed to track the cytokines secretion in PMNs (TNF-α, IL-8, IL-1β) as well as in human colon adenocarcinoma cell line Caco-2 (IL-8). The gene discussed is TNF; the disease is colon adenocarcinoma.