TWIST1 and cancer: The enriched cells were analyzed at the molecular level for the presence of epithelial cell lineage-specific markers (EpCAM and CK19), EMT-related markers (FAM83A, PTHLH, ERBB3, and TWIST1), CSC-related markers (NANOG, PROM1, and MET), the lung-specific marker BPIFA1, and the general cancer-related markers UCHL1 and GRP.