In conclusion, the current study demonstrated that 2′-HCA could induce apoptosis in human promyelocytic leukemia HL-60 cells via the ROS-dependent JNK pathway involving AP-1-DNA binding, which in turn may increase Bim protein expression, leading to mitochondrial translocation and the subsequent disruption of ΔΨm (Figure 9). This evidence concerns the gene JUND and acute promyelocytic leukemia.