CD4 and neoplasm: Furthermore, they showed that PCN–ACF–CpG@HA-based PDT treatment in vivo of H22 tumor-bearing mice resulted in a drastic tumor shrinkage associated with an increase in the number of mature DCs in tumor draining lymph nodes, increased expression of the IFN-γ, TNF-α, and IL-12p70 immune-related proinflammatory cytokines (secreted by DCs), and increased infiltration of tumor tissue by CD8+ and CD4+ lymphocytes [92].