In order to evaluate the effect of MMC NPs on the apoptosis of tumor cells, MB49 cells were cultured with MMC NPs, MMC RGD-NPs, and MMC solution for 24 h, then the cells were treated with Annexin V-FITC/PI, and the proportion of apoptotic cells was analyzed by flow cytometry. This evidence concerns the gene ANXA5 and neoplasm.