In conclusion, we synthesized and investigated a novel series of sulfonamides incorporating pyrazole- and pyridazinecarboxamide moieties for their effective inhibition against different and most relevant human carbonic anhydrase isoforms such as the ubiquitous hCA I, hCA II, and tumor associated isoforms hCA IX and XII, which are involved in a variety of diseases such as glaucoma, retinitis pigmentosa, epilepsy, and tumors. This evidence concerns the gene CYP24A1 and neoplasm.