Although anti-EGFR therapies are available for RAS wild-type CRC patients, and anti-VEGF, anti-VEGFR, recombinant fusion protein and multi-kinase inhibitor were applied in CRC patients with RAS mutation [102], unresponsiveness was seen in CRC patients with BRAF and PIK3CA mutations [103]. The gene discussed is PIK3CA; the disease is colorectal carcinoma.