In order to confirm the results, we established the MEKK5 knockdown cell lines in lung cancer NCI-H1650 cells and the MEKK5 overexpression cell lines in lung cancer A549 cells using a lentiviral expression system, and we detected the knockdown or the overexpression effect on the MEKK5 protein level. The gene discussed is MAP3K5; the disease is lung carcinoma.