For this analysis, we used three GIC cultures and found IC50s of 8.1 μM (BT48EF), 12.7 μM (BT12M), and 25.5 μM (GSCs-5), as shown in Figure 2E. Next, in order to determine the effects of SFX-01 on cell proliferation, we verified the expression of Ki67, by FACS, in the controls or after the administration of SFX-01 at 5, 10, and 20 μM in four GBM (U251, U87MG, T98G, and A172) cell lines and two GIC (BT12M and GSGs-5) cultures (Figure 3). The gene discussed is MKI67; the disease is glioblastoma.