So, we took Oct3/4 into consideration as an example for stem cell markers, CD44, CD90, and Stro1 for MES markers; β3-tubulin, neurofilaments (NF200), and nestin for neural markers; and GFAP as an astrocyte marker by using established glioma (U251, U87MG, A172 and T98G) cell lines and patient-derived GICs (BT12M). The gene discussed is NES; the disease is central nervous system cancer.