Focusing on specific routes of the immune response, it is worth highlighting the exclusive deregulation of the complement pathway after the infection with the double RNA2 mutant, upregulating c3 and the regulator of the complement cascade cfhr3 only at 2 days p.i. The early upregulation of c3 has been previously described in gilthead seabream and European seabass infected with betanodavirus [26]. This evidence concerns the gene C3 and infection.