A previous study showed that erythroid cells derived from patients’ iPSCs with pyruvate kinase deficiency (PKD), a rare metabolic blood disease caused by the mutation in PKLR gene, exhibited energetic imbalances such as decrease in adenosine triphosphate (ATP) levels and metabolites in the glycolysis pathway; the study also showed that the disease phenotypes were restored after knocking in the PKLR gene via TALEN-mediated homologous recombination [103]. This evidence concerns the gene PKLR and Hemolytic anemia due to red cell pyruvate kinase deficiency.