ATG7 and neoplasm: In tumor-derived cell lines, Atg7 depletion did not cause mitochondrial dysfunction, because the number of nonsynonymous mutations to trigger such an effect was too low; however, Atg7 deficiency did result in the reduction of metabolite recycling during starvation, particularly regarding TCA cycle intermediates including glutamate, aspartate, and α-ketoglutarate.