In fact, a study including only 32 cases [39] found that the association of lymph node metastases became significant when considering a ≥5% cut-off for PD-L1 expression (60% vs. 40%; p = 0.044), while a cut-off ≥1% did not reach statistical significance. This evidence concerns the gene CD274 and metastatic malignant neoplasm in the lymph nodes.