ESR1 and hepatocellular carcinoma: The significant late-stage upregulation of a subset of nine of these genes (ACADVL, FOXO1, SOCS2, NFKBIA, LCAT, MT1F, UBB, RHOB, ESR1) was verified in a tSNE embedding (Figure 5D), independently confirming a distinct cellular HCC subpopulation associated with an aging phenotype.