Confirming these findings in vivo, we observed that in orthotopic CCA mice a number of critical metabolic genes such as Pfkp, fatty acid synthase (Fasn), Atp6 (p < 0.001) and hexokinase 2 (Hk2) (p < 0.0001) are upregulated in tumor liver and metastatic liver LNs (Figure 5F). This evidence concerns the gene HK2 and cholangiocarcinoma.