These findings were also corroborated in the liver and liver LNs from orthotopic CCA animals that showed a high expression of EMT genes such as α-Sma, Fibronectin, Gli, Ptchd1, Twist1 as well as Vimentin indicating activation of the EMT pathways in vivo (Figure 1G). The gene discussed is ACTA1; the disease is cholangiocarcinoma.