To understand if CXCR2-CXCL5 signaling crosstalk alters CCA migration and metastasis, we performed the Boyden chamber assay and scratch wound assay with CCA cells treated with/without CXCL5 and CXCL5 + SB225002 for 24 and 48 h, respectively. The gene discussed is CXCL5; the disease is cholangiocarcinoma.