We found significant suppression of several focal adhesion genes such as Vinculin (VCL), Zyxin (ZYX), Talin1 (TLN1), Cadherin 8 (CDH8) (p ≤ 0.0001) in CCA cells treated with both inflamed LEC-CM as well as CXCL5, underscoring its role in this axis (Figure 4F,G). This evidence concerns the gene CDH8 and cholangiocarcinoma.